Single cell RNA sequencing allows the identification of active, transcribed genes on single cell level. This high resolution supports in depth insight into different cell types and states. We utilize this method mainly to understand T cell phenotypes (characteristics) throughout their development and in health and disease.
Human skin organoids are three dimensional (3D) cell cultures, composed of collagen based matrix populated with cells, isolated from human skin biopsies. Mimicking the 3D-architecture of a skin tissue allows a better recapitulation of a naive organ morphology and functionality. To investigate immune cells function and response in a diseased skin, we incorporate diseased associated immune cells into those skin organoids. Such immune-competent skin-cultures can be potentially employed as a testing platform for drug toxicity and efficacy studies, and as a tool to study immune cells function and response in a diseased skin.